human normal hepatic cell lines  (ATCC)

Journal: Cell Death Discovery

Article Title: pir-hsa-216911 inhibit pyroptosis in hepatocellular carcinoma by suppressing TLR4 initiated GSDMD activation

doi: 10.1038/s41420-024-02285-9

Figure Lengend Snippet: A The diagram illustrates our small RNA sequencing pipeline. B The heatmap displays the quality of reads based on their length. The read quality mainly concentrated at 38, ranging from 1 to 45 nt. C This histogram provides a visual representation of the distribution of sRNA tag length. Aside from the primary peak indicating miRNA (19–25), the secondary peak corresponds to piRNA (24–30). D The donut plot and list show sRNA tag mapping and annotation results. piRNAs represented only 1–3% of sequenced sRNAs. E Volcano plots show the distribution of differentially expressed genes in the three HCC cell lines compared to normal liver cells. F The Venn diagram shows the DEG counts in each HCC cell line. G The Venn diagram shows the DEG counts in each set. H Histogram display reads count distribution in all cell lines in sRNA sequencing result. I qPCR showed decreased pir-hsa-216911 expression in HCC cell lines compared to normal liver cells; ***p < 0.001, ** p < 0.01 by student-T tests against piRNA expression levels in HL7702.

Article Snippet: Human normal hepatic cell line HL7702 and hepatocellular carcinoma cell lines Huh7, HepG2, and Hep3B were obtained from the China Center for Type Culture Collection.

Techniques: RNA Sequencing, Sequencing, Expressing

Journal: Journal of Hepatocellular Carcinoma

Article Title: The Potential of the lncRNAs ADAMTSL4-AS1, AC067931 and SOCS2-AS1 in Peripheral Blood Mononuclear Cells as Novel Diagnostic Biomarkers for Hepatitis B Virus-Associated Hepatocellular Carcinoma

doi: 10.2147/JHC.S463804

Figure Lengend Snippet: The relative expression profile of the selected lncRNAs in CHB patients, HBV-associated LC patients, and HBV-associated HCC patients. Relative lncRNA expression levels in PBMC samples were detected by qRT-PCR. ( A ) ADAMTSL4-AS1, ( B ) AC067931, ( C ) SOCS2-AS1, ( D ) AL731577, ( E ) TPM-AS, ( F ) AL356356, ( G ) AC037198, ( H ) AP002026. The expression levels of related lncRNAs in liver tissues were detected by qRT-PCR. ( I ) ADAMTSL4-AS1, ( J ) AC067931, ( K ) SOCS2-AS1, ( L ) AL731577. Relative expression levels of lncRNAs were upregulated in four human HCC cells(Huh7, HepG2, Hep3B, HepG2.215) comparison with Lx2 cells determining by qRT-PCR. ( M ) ADAMTSL4-AS1, ( N ) AC067931, ( O ) SOCS2-AS1. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

Article Snippet: LX2 (human normal hepatic stellate cell line) and HCC (Huh7, HepG2, Hep3B, and HepG2.215) cell lines (Procell, Wuhan, China) were cultured under a 5% CO 2 atmosphere at 37°C, using Dulbecco’s Modified Eagle Medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Biological Industries, Israel), 1% streptomycin, and 1% penicillin solution (Gibco).

Techniques: Expressing, Quantitative RT-PCR, Comparison

Journal: Frontiers in Immunology

Article Title: Elevated MPP6 expression correlates with an unfavorable prognosis, angiogenesis and immune evasion in hepatocellular carcinoma

doi: 10.3389/fimmu.2023.1173848

Figure Lengend Snippet: MPP6 expression in HCC. (A) MPP6 mRNA expression in malignant tumor and normal tissues based on the TCGA database. (B, C) MPP6 mRNA expression in normal liver and HCC tissues based on GSE112791 (GPL570 platform) and GSE101685 datasets. (D) MPP6 mRNA expression in HCC cell lines (Huh7, Hep3B, BEL-7404, and SMMC-7721) compared with the normal hepatic cell line LO2 by qRT−PCR. (E) MPP6 protein expression in normal liver and HCC tissues based on the UALCAN website. (F) MPP6 protein expression in LO2 and HCC cell lines (Huh7, Hep3B, BEL-7404, and SMMC-7721) by Western blotting. (G) Immunohistochemical staining analysis of MPP6 in normal liver and HCC tissues. * P < 0.05; ** P < 0.01; *** P < 0.001; ns: P > 0.05.

Article Snippet: The human normal hepatic cell line LO2 was provided by Nanjing KeyGen Biotech.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Staining